University of Bristol
First Class Honours (2019)
Information about Firat
My name is Firat and I graduated from University of Bristol in 2019, achieving a First Class Honours in Biochemistry. I achieved AAA at A Levels which included Chemistry, Biology and Economics. I am excited to share my knowledge and expertise with other A Level students. I am currently working as a Scientist at a Pharmaceutical Company.
I look forward to hearing from you.
Willing to travel: Home Only
Experience: BSc (Hons), Biochemistry, September 2016 - June 2019
First Class Honours, University of Bristol
Main subjects studied:
Advanced biochemistry (71%), advanced cell biology (74%), the dynamic proteome (73%), cellular composition (84%), Biological Chemistry 1B powering biomolecular interactions (73%)
Final year project (graded 76%)
My project outlines how the conjugation of synthetic, fusogenic, GALA peptide to the exterior of self-assembling peptide cages (SAGEs) which encase therapeutic compounds, can facilitate their endosomal escape.
Evaluated the use of GALA by exploring the issue of endosomal entrapment of SAGE particles through the available literature, critically analysing information pertaining to this problem, such as the current methods involving the proton-sponge effect.
Planned the use of amino acid sequence information for total gene synthesis of SAGE-particle component CC-Tri, GALA, SEG-Linker and His/Flag tag fusions. The resulting DNA sequences are optimised for expression in the E. Coli strain BL21-AI. Polymerase-chain-reaction (PCR), ligation-independent cloning and transformation of E. Coli using expression vectors allowed GALA-SEG-CC-Tri fusion protein expression.
Overall aim of quantifying the endosomal escape of GALA-SAGE particles, which was done using the real-time split-GFP fluorescence complementation assay.
A-Levels, September 2014 June 2016
A Levels: Biology (A), Economics (A), Chemistry (A)
Formulation Scientist, May 2020 Present
Using my initiative, proven data-driven approaches and following standard operating protocols as a scientist at Catalent, I am involved in developing drug candidates into first-in-man studies and advancing them into formulated products. My role is predominantly laboratory based; where I plan and execute feasibility studies to produce GLP pharmaceutical products which involves operating a series of high-tech equipment, such as the viscometer. I am also responsible for presenting experimental findings to clients as well as compiling technical documents on the operation of equipment, such as the particle size analyser.