Producing Monoclonal Antibodies (biology Only) (GCSE Biology)

Why are hybridoma cells cloned (for example by limiting dilution) after the correct antibody-producing cell has been identified?

A diagnostic lab wants to ensure a monoclonal antibody test does not give false positives due to cross-reaction. Which approach would best check specificity?

What is the main reason scientists immunise a mouse with an antigen before making monoclonal antibodies?

What does the term “epitope” refer to in monoclonal antibody production?

A lab develops a monoclonal antibody test strip for a virus. Which control should be included to show the test reagents and flow are working correctly?

If a monoclonal antibody used in a diagnostic strip is enzyme-labelled, what will happen at the test area when the antibody–antigen complex is present?

Which laboratory method is commonly used to purify monoclonal antibodies from culture supernatant?

Which of the following best describes a monoclonal antibody?

During hybridoma production, why might researchers perform ELISA (enzyme-linked immunosorbent assay) on culture supernatants?

In a lateral flow test (test strip) using monoclonal antibodies, what is the primary purpose of the control line?

How can monoclonal antibodies be modified to detect multiple different targets in a single lab assay?