Producing Monoclonal Antibodies (biology Only) (GCSE Biology)

In a lateral flow test (test strip) using monoclonal antibodies, what is the primary purpose of the control line?

What is the purpose of using a selection medium (e.g., HAT medium) after fusing lymphocytes and myeloma cells?

What name is given to the fused cell that can both divide indefinitely and produce a specific antibody?

Why are hybridoma cells cloned (for example by limiting dilution) after the correct antibody-producing cell has been identified?

If a monoclonal antibody used in a diagnostic strip is enzyme-labelled, what will happen at the test area when the antibody–antigen complex is present?

A diagnostic lab wants to ensure a monoclonal antibody test does not give false positives due to cross-reaction. Which approach would best check specificity?

Which label is frequently attached to monoclonal antibodies to make antigen–antibody binding visible in diagnostic tests?

Which property of monoclonal antibodies makes them especially useful for diagnostic tests?

Which ethical concern is most often raised about traditional methods of producing monoclonal antibodies using mice?

Why must aseptic technique be used when culturing hybridoma cells to produce monoclonal antibodies?

Which method is commonly used to identify which hybridoma clones make the desired antibody?