Microscopy (GCSE Biology)

When using a light microscope, which adjustment knob should you use first to bring your specimen roughly into focus?

You view a cell image that measures 4.8 cm on the microscope image at ×400 total magnification. What is the real length of the cell in micrometres (µm)?

Which formula gives the total magnification when using a light microscope with an eyepiece lens and an objective lens?

Why is magnification not the same as resolving power?

Why is it important to use a very thin layer of epidermis when making a slide from onion tissue?

Which feature of onion epidermal cells makes them especially suitable for study under a light microscope?

Which microscope type would you choose if you need to see the surface topography and 3D shape of a specimen at high resolution?

Which of the following organelles could you expect to see clearly in a plant cell using a standard light microscope with stain?

When preparing a wet mount, a pupil gently blots the excess liquid from around the cover slip. What is the primary reason for this?

During calibration of an eyepiece graticule, an image of a stage micrometer shows that 1 division on the graticule = 10 µm at ×100 magnification. How many micrometres does 5 divisions represent at the same magnification?

When drawing cells observed under a microscope for assessment, which practice is recommended?